Freeze-drying of foot and mouth disease virus and its application in inactivated virus vaccine production
نویسنده
چکیده
Five different subtypes of foot and mouth disease (FMD) virus propagated in BHK21 C13 monolayer cell cultures, namely 05, A5/!0, A22, C¡ and Asia 1/1, were used to study the stability of live virus during freeze-drying and subsequent storage. Binary ethyleneimine-inactivated FMD virus vaccines adjuvanted with purified saponin, "Quil-A", without aluminium hydroxide gel, were examined before and after freeze-drying and storage for potency in guinea pigs by challenge tests and in cattle by neutralising antibody estimation. The infectivity titres of virus subtypes A5/!0, A22, C1 and Asia 1/1 were observed to be retained during the freeze-drying process and with minimal loss when stored at —20°C for one year. The infectivity of 05 virus was found comparatively less stable. The complement fixing (CF) antigen titres of all virus subtypes remained unaffected under these conditions. The test results in guinea pigs and cattle indicated that the freeze-dried inactivated vaccines of virus subtypes A5/10, A22, C1 and Asia 1/1 adjuvanted with "Quil-A " alone were potent, and that they retained their potency after storage at -20°C for at least one year.
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تاریخ انتشار 2009